An Improved Fluorescent Amplified Fragment Length Polymorphism Method for Typing Mycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis.
We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of...
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Whole-genome fingerprinting fluorescent amplified fragment length polymorphism (FAFLP) data were compared with in silico data for the sequenced strains of Mycobacterium tuberculosis (H37Rv and CDC1551). For this G+C-rich genome, many predicted fragments were not detected experimentally. For H37Rv, only 108 (66%) of the 163 predicted EcoRI-MseI fragments between 100 and 500 bp were visualized in...
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Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis is a recently developed technique which allows strains of tuberculosis to be differentiated with a high degree of confidence. The application of this technique has enabled the patterns of transmission of tuberculosis to be elucidated with far greater certainty than was previously possible. This review will disc...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.44.1.288-289.2006